BIO GENETICS LAB 2 QUESTIONS HELP

profile aslan

  • Master

NEED HELP WITH Blank BOX QUESTION

(question i and 4 in conclusion role. )

* It would be dainty if you tin check my answeres besides.

NOTES:

Tube Sample Lane

1 i kb Dna ladder 1

2 Mother's DNA two

three Child'south DNA 3

iv A.F. #1 DNA 4

5 A.F. #ii DNA 5

Record the distance each ladder band moved from the well in mm along with the size of the Dna fragments in that band in bp units, based on the bp given in stride 19 , in your Lab Notes.

Lane one:

one,000 bp 34 mm

900 bp 36 mm

800 bp 38 mm

700 bp 40 mm

600 bp 43 mm

500 bp 47 mm

400 bp 51 mm

300 bp 56 mm

250 bp 60 mm

200 bp 64 mm

150 bp 69 mm

100 bp 77 mm

50 bp xc mm

Lane 2:

37 mm 850bp

59 mm 265bp

Lane 3:

37 mm 850bp

44 mm 575bp

Lane 4:

41 mm 670bp

43 mm 600bp

Lane 5:

44 mm 575bp

55 mm 320bp

Experiment: Agarose Gel Electrophoresis of DNA Fragments

Lab Results

  1. List the distances traveled in mm for the bands in the DNA ladder in the table beneath.
    Remember, smaller fragments travel further than longer ones, so the pinnacle-virtually ring will be the 1,000 bp sized DNA fragments whereas the bottom-most ring will exist the 50 bp sized DNA fragments.
    DNA Ladder
    Ring Distance (mm)
    50 bp  90 mm
    100 bp  77 mm
    150 bp  69 mm
    200 bp  64 mm
    250 bp  60 mm
    300 bp  56 mm
    400 bp  51 mm
    500 bp  47 mm
    600 bp 43 mm
    700 bp 40 mm
    800 bp 38 mm
    900 bp 36 mm
    1,000 bp 34 mm
  2. Whose sample had the approximately 570 bp and 320 bp sized Dna fragments?
  3. What were the sizes of the Deoxyribonucleic acid fragments for alleged father #1?

Information Assay

  1. Which size DNA fragment did the kid inherit from her mother?
  2. Which alleged male parent, if whatsoever, can be definitively ruled out as the kid'southward biological father?

Conclusions

  1. How are new molecules of DNA synthesized in living cells?
  2. What is the function of Dna?

    DNA has genetic data that controls our cells. And then, DNA is like a blueprint that shows how to construct components of cells similar proteins and ribonucleic acid (RNA). This information is carried downward to newer generations through inheritance.

  3. If each individual has such a small amount of Deoxyribonucleic acid in their cells, how do the bands on the gel contain enough DNA to be visible?

      In order to make DNA visible. The Gel has to be soaked in a dye (ethidium bromide) to bind with the Dna and rinsed off after. Ethidium bromide helps to make DNA visible past glowing brightly in UV rays.

  4. Humans just have a few eye colors and only 4 ABO-based claret types. How can Deoxyribonucleic acid tests definitively identify individuals when many people accept brown eyes or type A blood?
  5. Suppose a suspicious hair was found in a victim's home. A gel is set with the Dna fragments of ii suspected criminals in lanes 4 and v, the DNA fragments of the suspicious hair in lane three, and the victim's Deoxyribonucleic acid fragments, as a negative control, are in lane 2. A DNA ladder is in lane 1. The resulting gel is below. Which suspect, if any, committed the crime? Explain your respond.

     Lane 4,5 (two suspected criniminals)

  • attachment

    asfdaffdsf.png

Answer( 1 )

Purchase the answer to view it

blurred-text

NOT RATED

  • attachment

    here.docx

class=css-xly9o6